Chuenboonngarm, N, et al., ScienceAsia 27: 137-141 (2001)
Summary
Shoot tips of Gardenia jasminoides Ellis were successfully propagated in B5 agar medium with BA (0, 2.5, 5, 7.5 and 10 mg/l) and 2iP (0, 2.5, 5, 7.5 and 10 mg/l) concentrations. The number of shoots in medium with 10 mg/l BA was 7 times greater than those in medium without BA. The BA induced plantlets had 100% survival rate and normal growth rate ex-vitro. The number of shoots in medium with 7.5 mg/l 2iP was 4 times greater than in 2iP free medium. All explants receiving 2iP gave chimeric plants showing different degrees of white leaf tissue. This is the first report of somaclonal variation induced by 2iP in G. jasminoides. Chimeric plants had 70% survival rate and showed slow growth ex-vitro.
Objectives
This investigation was to evaluate the use of standard medium B5 in G. jasminoides tissue culture.
Materials
Young shoots of Gardenia jasminoides Ellis, 5 cm in length, were obtained from 5 plants grown in natural condition, one from Suan Luang Rama IX Botanic Garden, Bangkok, Thailand, the others from Faculty of Science, Mahidol University, Bangkok, Thailand.
Explant
Shoot tips collected from the same mother plant were divided for two different cytokinin treatments each time cultures were initiated. After leaf removal, selected shoots were washed with mild detergent and rinsed in tap water for 10 min. The shoots were then soaked in 15% (v/v) Clorox in 0.25% (v/v) Tween-20 solution. After 10 min, the shoots were soaked for 10 min in 10% (v/v) Clorox in 0.25% (v/v) Tween-20 solution, followed by five 5-min rinses in sterile distilled water. Excised shoot tips, 0.5-1.0 cm in length, were used as explants.
Initial culture
B5 agar medium with 10 mg/l BA
Shoot multiplication
B5 agar medium with 7.5 mg/l 2iP
Rooting
Root induction was achieved by transferring new shoots to B5 agar medium with no plant growth regulator.
Acclimation
Plantlets with roots were transplanted to damp vermiculite in containers with clear lids under the same environment as those in cultures. Plantlets　were acclimatized to normal environment by replacing the container lids with colorless transparent plastic sheets with 1 mm pinholes for 1 month.
Planting
Plantlets were then potted and kept in greenhouse under 50% natural light. All were transferred outdoors after 2 months.