Genetical descrimination

method

Sequencing(direct)

level

種 (species)

result_info

Paeonia lactiflora can be discriminated by the polymorphism of ITS region.

Comments

primer sequence (5'-3') forward TCC ACT GAA CCT TAT CAT TTA G TCT CGC ATC GAT GAA GAA CG reverse CCA TGC TTA AAC TCA GCG GGT CCA TGC TTA AAC TCA GCG GGT

Related Crude drug data

シャクヤク

Gene data

Genetical descrimination results

Result file	3_alignment-シャクヤク.gif
Pubmed ID
References	Sang,T., Crawford,D.J. and Stuessy,T.F. Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution Proc. Natl. Acad. Sci. U.S.A. 92, 6813-6817 (1995) Pan,J., Zhang,D. and Sang,T. Molecular phylogenetic evidence for the origin of a diploid hybrid of Paeonia (Paeoniaceae) Am. J. Bot. 94 (3), 400-408 (2007) Molecular phylogenetic evidence for the origin of a diploid hybrid of Paeonia (Paeoniaceae) Sun,Y.L. and Hong,S.K. Genetic diversity and phylogenetics of genus Paeonia based on nuclear ribosomal DNA ITS sequence J. Plant Biotechnol 38, 234-240 (2011) Punina,E.O., Machs,E.M., Krapivskaya,E., Kim,E.S., Mordak,E.V., Myakoshina,Y.A. and Rodionov,A.V. Interspecies hybridization in the genus Paeonia (Paeoniaceae): polymorphic sites in the transcribed spacers of the 45S rRNA genes as an indicator of origin of natural and garden peony hybrids Russ. J. Genet.48(7),684-697,2012
Comments

Comprehensive Medicinal Plant Database

Genetical descrimination