| method | Sequencing(direct) |
| level | 種 (species) |
| result_info | Pueraria lobata can be discriminated from P. thomsonii and P. montana by the polymorphism of ITS2 region. |
| Comments | primer sequence (5'-3')
forward GCA TCG ATG AAG AAC GTA GCG
reverse GTA GTC CCG CCT GAC CTG
|
| Related Crude drug data | カッコン |
| Gene data |
PUERARIAE RADIX(ITS2,DQ302142)
,
PUERARIAE RADIX(ITS2,DQ302143)
PUERARIAE RADIX(ITS2,DQ302146)
,
PUERARIAE RADIX(ITS2,DQ302149)
PUERARIAE RADIX(ITS2,DQ472517)
,
PUERARIAE RADIX(ITS2,NIB-0542)
PUERARIAE RADIX(ITS2,NIB-0543)
,
PUERARIAE RADIX(ITS2,NIB-0257)
PUERARIAE RADIX(ITS2,NIB-0552)
,
PUERARIAE RADIX(ITS2,NIB-0634)
PUERARIAE RADIX(ITS2,NIB-0454)
,
PUERARIAE RADIX(ITS2,NIB-0535)
PUERARIAE RADIX(ITS2,NIB-0536)
,
PUERARIAE RADIX(ITS2,NIB-0537)
PUERARIAE RADIX(ITS2,NIB-0548)
,
PUERARIAE RADIX(ITS2,NIB-0538)
PUERARIAE RADIX(ITS2,NIB-0540)
,
PUERARIAE RADIX(ITS2,NIB-0541)
PUERARIAE RADIX(ITS2,NIB-0413)
,
PUERARIAE RADIX(ITS2,NIB-0414)
PUERARIAE RADIX(ITS2,NIB-0534)
,
PUERARIAE RADIX(ITS2,NIB-0539)
PUERARIAE RADIX(ITS2,NIB-0544)
,
PUERARIAE RADIX(ITS2,NIB-0545)
PUERARIAE RADIX(ITS2,NIB-0551)
,
PUERARIAE RADIX(ITS2,NIB-0746)
|
Genetical descrimination results  |
| Result file | alignment fig-kakkon-db.tif |
| Pubmed ID | |
| References | Y. Sun, P. C. Shaw, K. P. Fung, Molecular authentication of Radix Puerariae Lobatae and Radix
Puerariae Thomsonii by ITS and 5S rRNA spacer sequencing, Biol. Pharm. Bull., 30, 173-175 (2007). |
| Comments | Pueraria lobata, P. thomsonii, and P. montana can be discriminated. |
|