| method | Sequencing(direct) |
| level | 系統 (line, strain) |
| result_info | Discrimination of Pueraria lobata, P. thomsonii, and P. montana by polymorphism of 5S-rDNA intergenic spacer region. |
| Comments | primer sequence (5'-3')
forward "GGA TCC GTG CTT GGG CGA GAG TAG TA"
reverse "GGA TCC TTA GTG CTG GTA TGA TCG CA"
Ref.) Y. Sun, P. C. Shaw, K. P. Fung, Molecular authentication of Radix Puerariae Lobatae and Radix
Puerariae Thomsonii by ITS and 5S rRNA spacer sequencing, Biol. Pharm. Bull., 30, 173-175 (2007). |
| Related Crude drug data | カッコン |
| Gene data |
PUERARIAE RADIX(5S rDNA,DQ472521)
,
PUERARIAE RADIX(5S rDNA,DQ472522)
PUERARIAE RADIX(5S rDNA,DQ302154)
,
PUERARIAE RADIX(5S rDNA,DQ302155)
PUERARIAE RADIX(5S rDNA,DQ302156)
,
PUERARIAE RADIX(5S rDNA,DQ302157)
PUERARIAE RADIX(5S rDNA,DQ472519)
,
PUERARIAE RADIX(5S rDNA,DQ472520)
PUERARIAE RADIX(5S rDNA,DQ485980)
,
PUERARIAE RADIX(5S rDNA,DQ302150)
PUERARIAE RADIX(5S rDNA,DQ302151)
,
PUERARIAE RADIX(5S rDNA,DQ302152)
PUERARIAE RADIX(5S rDNA,DQ302153)
,
PUERARIAE RADIX(5S rDNA,DQ485981)
PUERARIAE RADIX(5S rDNA,DQ472523)
,
PUERARIAE RADIX(5S rDNA,DQ485982)
|
Genetical descrimination results  |
| Result file | electropherogram-1.tif |
| Pubmed ID | |
| References | |
| Comments | Electropherogram of NIB-257, NIB-413, NIB-414, NIB-454, and NIB-534.
primer sequence (5'-3')-1st round
forward GG AAG TCC TCG TGT TGC AC
reverse CAC GGG GCC CAC AAT AC
NIB-257: genotype BX
NIB-413, 414: genotype BB
NIB-454: genotype BC
NIB-534: genotype AB(D) |
| Result file | electropherogram-2.tif |
| Pubmed ID | |
| References | |
| Comments | Electropherogram of NIB-535, NIB-536, NIB-537, NIB-538, and NIB-539.
primer sequence (5'-3')-1st round
forward GG AAG TCC TCG TGT TGC AC
reverse CAC GGG GCC CAC AAT AC
NIB-535: genotype BC
NIB-536: genotype AX(D)
NIB-537: genotype CC
NIB-538: genotype AB(D)
NIB-539: genotype BB |
| Result file | electropherogram-3.tif |
| Pubmed ID | |
| References | |
| Comments | Electropherogram of NIB-540, NIB-541, NIB-542, NIB-543, and NIB-544.
primer sequence (5'-3')-1st round
forward GG AAG TCC TCG TGT TGC AC
reverse CAC GGG GCC CAC AAT AC
NIB-540, 543: genotype BB
NIB-541, 542: genotype BC
NIB-544: genotype AB(D)
|
| Result file | electropherogram-4.tif |
| Pubmed ID | |
| References | |
| Comments | Electropherogram of NIB-545, NIB-546, NIB-547, NIB-548, and NIB-551.
primer sequence (5'-3')-1st round
forward GG AAG TCC TCG TGT TGC AC
reverse CAC GGG GCC CAC AAT AC
NIB-545, 546, 548: genotype BC
NIB-547: genotype BX
NIB-551: genotype CD |
| Result file | electropherogram-5.tif |
| Pubmed ID | |
| References | |
| Comments | Electropherogram of NIB-552, NIB-634, NIB-746, and no template control.
primer sequence (5'-3')-1st round
forward GG AAG TCC TCG TGT TGC AC
reverse CAC GGG GCC CAC AAT AC
NIB-552: genotype BC
NIB-634: genotype CC
NIB-746: genotype BB
|
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