Tissue Culture Literation
| Plant latin name | Zanthoxylum piperitum (L.) DC. |
| Literature code | Zanthoxylum_piperitum-Ref-2 |
| Reference | Imaizumi K., et al., Biosci. Biotech. Biochem.，57 (4)，591-593 (1993) |
| Summary | An analysis of the aroma components of Asakura-sanshoo leaves was conducted by using head-space gas chromatography. It shows that a greater part of the shoot-tip culture Iines of Asakura-sanshoo preserved the same characteristics as those of the parent plant with respect to aroma productivity in the secondary metabolites，apart from a few variant strains. It was found ，however，that the level oftotal aroma components of selected variant culture lines gradually increased during five subcultures，although that of the control culture lines remained low. Thus ，the combination of shoot-tip culture and selection was found to be very effective for improving Asakura-sanshoo with high aroma emanation. |
| Objectives | In this paper，an aroma analysis of different leaves from plant lines derived from a shoot-tip culture of Asakura sanshoo，from the parent plant and from seedlings is reported. |
| Materials | |
| Explant | Meristem tissues cut from the axillary and apical buds of sterilized shoot segments |
| Initial culture | Incubation on modified MS medium with 3.0% sucrose and 0.9% agar containing lμM NAA and 1μM BAP under light conditions (2000Iux). |
| Shoot multiplication | |
| Rooting | |
| Acclimation | Rooted shoots of 5 to 10 cm height were transplanted to pots and grown in a greenhouse |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |