Tissue Culture Literation
| Plant latin name | Citrus unshiu Marcowicz |
| Literature code | Citrus_unshiu-Ref-2 |
| Reference | Masashi YAMAMOTO and Shozo KOBAYASHI, Plant Tissue Culture Letters，12 (2) ，131-137 (1995) |
| Summary | Electrofusion was conducted to combine Citrus unshiu cv. 'Juman' unshiu protoplasts isolated from embryogenic callus with C. sinensis cv. 'F. N. Washington' navel orange mesophyll protoplasts. One plant was regenerated from the fusion products. The plant has 18 chromosomes(2n = 18 in each parent) ，and it showed the same nuclear rDNA fragment pattern as that of C. sinensis. Whereas，chloroplast and mitochondrial DNA analyses showed that the plant contained C. unshiu chloroplast and mitochondrial genomes. From these results， the regenerated plant was confirmed as a cybrid having C. sinensis nuclear genome and C. unshiu cytoplasmic genome. |
| Objectives | We report here the production of a cybrid having cytoplasmic genome of C. unshiu which probably shows sterile cytoplasm and nuclear genome of C. sinensis. |
| Materials | in vitro plants |
| Explant | leaves and callus |
| Initial culture | Maintaining for one year on agar medium consisting of Murashige and Tucker (MT) medium with 20 mg/l kinetin. |
| Shoot multiplication | A green embryoid derived from protoplasts was transferred to BM containing 500 mg/l malt extract，40 mg/l adenine，5% sucrose，and 0.9% agar. Cotyledonary embryoid which developed were transferred to BM containing 10 mg/l gibberellic acid，2% sucrose，and 0.9% agar. |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |