Comprehensive Medicinal Plant Database

Tissue Culture Literation

Plant latin name Aconitum carmichaeli Debeaux
Literature code Aconitum_carmichaeli-Ref-2
Reference C. SHIPING S. J. SHAN,H. TANAKA and Y. SHOYAMA, BIOTRONICS 27,15-20,1998
Summary The clonally propagated Aconitum carmichaelii Debx. was used for the investigation of microtubering. The rooting condition of propagated shoots affected the establishment of transplantation to soil indicating the Murashige-Skoog medium supplemented with 0.5 mg/l of IAA and the cultivation at 20oC are the best. Culturing at 15°C under the dark enhanced microtubering rather than 10oC or 20oC. The temperature affected the production of aconitine-type alkaloids, demonstrating that the contents of mesaconitine and hypaconitine were higher at 20oC than at 15 and 10oC.
Objectives The effect of culturing temperature on the establishment of transplantation to soil,the microtuber formation from the clonally propagated shoots and the aconitine-type alkaloid content were investigated.
Materials Clonal A. carmichaelii plants were produced by shoot tip culture
Explant In vitro shoots
Initial culture
Shoot multiplication Rooted plantlets were cultured under the dark condition in the growth cabinet at 15oC for 6 weeks to induce microbubers.
Rooting MS medium supplemented with IAA 0.5mg/L in 16 h light from cool white fluorescent tubes (20001ux at 20 士 10oC for 4 weeks.
Acclimation Stored microtubers in a refregerator (4oC) for 3 months were cultivated at 20oC.
Planting
Cultivation conditions
Traints of regenerants
Ingredients analyzed Mesaconitine, aconitine and hypaconitine
Extraction HPLC system
Analitical methods
Notes