Comprehensive Medicinal Plant Database

Tissue Culture Literation

Plant latin nameAconitum carmichaeli Debeaux
Literature codeAconitum_carmichaeli-Ref-2
ReferenceC. SHIPING S. J. SHAN,H. TANAKA and Y. SHOYAMA, BIOTRONICS 27,15-20,1998
SummaryThe clonally propagated Aconitum carmichaelii Debx. was used for the investigation of microtubering. The rooting condition of propagated shoots affected the establishment of transplantation to soil indicating the Murashige-Skoog medium supplemented with 0.5 mg/l of IAA and the cultivation at 20oC are the best. Culturing at 15°C under the dark enhanced microtubering rather than 10oC or 20oC. The temperature affected the production of aconitine-type alkaloids, demonstrating that the contents of mesaconitine and hypaconitine were higher at 20oC than at 15 and 10oC.
ObjectivesThe effect of culturing temperature on the establishment of transplantation to soil,the microtuber formation from the clonally propagated shoots and the aconitine-type alkaloid content were investigated.
MaterialsClonal A. carmichaelii plants were produced by shoot tip culture
ExplantIn vitro shoots
Initial culture
Shoot multiplicationRooted plantlets were cultured under the dark condition in the growth cabinet at 15oC for 6 weeks to induce microbubers.
RootingMS medium supplemented with IAA 0.5mg/L in 16 h light from cool white fluorescent tubes (20001ux at 20 士 10oC for 4 weeks.
AcclimationStored microtubers in a refregerator (4oC) for 3 months were cultivated at 20oC.
Cultivation conditions
Traints of regenerants
Ingredients analyzedMesaconitine, aconitine and hypaconitine
ExtractionHPLC system
Analitical methods