Tissue Culture Literation
| Plant latin name | Aconitum carmichaeli Debeaux |
| Literature code | Aconitum_carmichaeli-Ref-2 |
| Reference | C. SHIPING S. J. SHAN，H. TANAKA and Y. SHOYAMA, BIOTRONICS 27，15-20，1998 |
| Summary | The clonally propagated Aconitum carmichaelii Debx. was used for the investigation of microtubering. The rooting condition of propagated shoots affected the establishment of transplantation to soil indicating the Murashige-Skoog medium supplemented with 0.5 mg/l of IAA and the cultivation at 20oC are the best. Culturing at 15°C under the dark enhanced microtubering rather than 10oC or 20oC. The temperature affected the production of aconitine-type alkaloids, demonstrating that the contents of mesaconitine and hypaconitine were higher at 20oC than at 15 and 10oC. |
| Objectives | The effect of culturing temperature on the establishment of transplantation to soil，the microtuber formation from the clonally propagated shoots and the aconitine-type alkaloid content were investigated. |
| Materials | Clonal A. carmichaelii plants were produced by shoot tip culture |
| Explant | In vitro shoots |
| Initial culture | |
| Shoot multiplication | Rooted plantlets were cultured under the dark condition in the growth cabinet at 15oC for 6 weeks to induce microbubers. |
| Rooting | MS medium supplemented with IAA 0.5mg/L in 16 h light from cool white fluorescent tubes (20001ux at 20 士 10oC for 4 weeks. |
| Acclimation | Stored microtubers in a refregerator (4oC) for 3 months were cultivated at 20oC. |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | Mesaconitine， aconitine and hypaconitine |
| Extraction | HPLC system |
| Analitical methods | |
| Notes |