A. Zafarullah,S. Ilyas, S. Naz, F. Aslam and F. Manzoor, Pakistan Journal of Science 65(4): 462-466 (2013)
Summary
A rapid shoot multiplication of Chrysanthemum(Chrysanthemum indicum L.) has been optimized in the present study. Apical meristems were used as explants and inoculated on MS media supplemented with various concentrations of growth regulators. The effects of different phytohormones (BAP, NAA, IAA and IBA) were tested for their effect on enhancement of shoot multiplication. MS medium fortified with BAP 1.0 + IAA 0.1 mg/l had a promotory effect on the initiation and multiplication of micro shoots. The optimum shoot formation response was 82% with 5.20 average number of shoots per explants and acquired maximum length of 4.9 cm. Rooting study revealed that MS medium supplemented with IBA 0.1 mg/l were proved to be superior with maximum (85%) rooting, 2.7 roots per explants and 2.5 cm root length. Then plantlets were shifted in Green house for acclimatization.
Objectives
A rapid shoot multiplication of Chrysanthemum(Chrysanthemum indicum L.) has been optimized in the present study.
Materials
Chrysanthemum plants were collected from the Lawrence garden and Botanical garden of Lahore College for Women University (LCWU), Lahore Pakistan
Explant
For surface sterilization all apical meristem were taken from upper portions of the plants. After removing the leaves, apical meristems were separated with the help of fine scissor and washed with tap water for several times to remove the dust particles without damaging the young and delicate tissues. Then explants were washed and cleaned with detergent and later on rinsed with distilled water to remove traces of detergent. At the end, all the explants were dipped in commercial Sodium Hypochlorite solution (15-20%) for 15-20 minute. After this treatment, the explants were rinsed with autoclaved distilled water four-five times, so as to remove and lower the toxic effect of Sodium Hypochlorite.
Initial culture
MS medium supplemented with BAP 1 mg/l+ IAA 0.1 mg/l
Shoot multiplication
MS medium supplemented with BAP 1 mg/l+ IAA 0.1 mg/l
Rooting
MS medium supplemented with IBA 0.1 mg/l
Acclimation
Plantlets were shifted in green house for
acclimatization.