| Plant latin name | Paeonia lactiflora Pallas |
| Literature code | Paeonia_lactiflora-Ref-9 |
| Reference | Gabryszewska E., Journal of Fruit and Ornamental Plant Research, 18(2):309-320 (2010) |
| Summary | Herbaceous peony plants successfully propagated in vitro do not survive the
transfer to the ex vitro environment. For other species, storage organ formation in
vitro can limit the loss of plants during acclimatization. In the natural conditions, the renewal buds for the following year originate on the underground crown (metamorphosed underground shoot, rhizome) of herbaceous peony. A perennial crown and roots serve for the accumulation of the storage products and plant renewal. The aim of the experiment was to investigate the influence of glucose (30, 60, 90 g l-1) and growth regulators (kinetin, IBA, GA3) on the shoot, renewal bud, root growth and development of Paeonia lactiflora 'Jadwiga’ in vitro. Excision of all leaves from isolated explants inhibited production of new shoots and leaves, and evidently induced formation of renewal buds. Increasing the glucose supply, especially in the absence of growth regulators, decreased production of shoots and outgrowth of leaves. The stronger inhibition of shoot growth by glucose was observed on the explants without leaves. By contrast, the beneficial effect of glucose on renewal
bud formation was observed. A single supply of kinetin, IBA or GA3 stimulated
shoots and leaf growth and inhibited renewal bud formation, on the explants isolated
with leaves. Interaction of kinetin, GA3 and IBA (added together) and the highest
glucose level enhanced the growth of shoots on the explants containing leaves, and
increased the number of renewal buds, on the explants without leaves. Increasing
glucose level enhanced the number of roots in the absence of growth regulators on the
explants containing leaves. The supply of IBA in the medium containing 30 g l-1
glucose, stimulated the root production on the explants without leaves. The addition
of GA3 or kinetin (singly or simultaneously with IBA) to the medium with different
concentrations of glucose, strongly inhibited rooting. The results presented here, show that a high level of glucose and exogenous
growth regulators (kinetin, GA3, IBA) together stimulate shoot and renewal bud formation but the way of organogenesis depends on the presence or absence of leaves. The interaction between auxin (exogenous or endogenous) and glucose regulate root formation on the peony shoots but the final effect depends on the type of explants (with or without leaves). It is possible that leaves have a very important hormonal factors, which stimulate shoot growth or rooting and inhibit renewal bud formation. |
| Objectives | To investigate the influence of glucose (30, 60, 90 gl-1) and growth regulators (kinetin, IBA, GA3) on the shoot, renewal bud, root growth and development of Paeonia lactiflora ’Jadwiga’ in vitro |
| Materials | Paeonia lactiflora ’Jadwiga’ |
| Explant | The pieces of crown with one shoot (with or without leaves) excised from Paeonia lactiflora 'Jadwiga' propagated on the Murashige and Skoog (MS) medium containing kinetin 1 mgl-1 + 2iP 1 mgl-1 + BAP 1 mgl-1 + TDZ 0.01 mgl-1 and sucrose 30 gl-1. |
| Initial culture | Plant material used for the experiment (Paeonia lactiflora ‘Jadwiga’) was
propagated on the MS medium containing: kinetin 1 mgl-1 + 2iP 1 mgl-1 +
BAP 1 mgl-1 + TDZ 0.01 mgl-1 and
sucrose 30 gl-1 |
| Shoot multiplication | Two different inocula (the pieces of crown with one shoot with or without leaves) were used. The influence of glucose (30, 60, 90 gl-1) and kinetin 1 mgl-1, GA3 1 mgl-1,
IBA 1 mgl-1 (added to the medium alone or together) on the shoot, renewal bud and root formation was investigated. Culture conditions were a 16 h photoperiod provided by coolwhite fluorescent lamps (Philips
TLD 36W/95) at 80 μmolm-2s-1 and a temperature of 25 °C. Excision of all leaves promoted the renewal bud formation in the absence of exgenous growth regulators as well as in their presence in the medium. The renewal bud formation was induced by glucose on a growth regulator-free medium. Interaction of all growth regulator (kinetin, GA3, IBA added together) and high glucose level induced renewal bud formation (about 4.5/inoculum), on the inocula without leaves. The addition of 1 mgl-1 kinetin, IBA or GA3 (singly or together) inhibited renewal bud formation on the inocula with leaves. |
| Rooting | Leaves as the source of endogenous growth regulators influenced root formation. Inocula with leaves produced roots on the growth regulator-free medium but with the presence of glucose. Increasing glucose level strongly enhanced the number of roots (from 0.4 to 2.6 roots/inoculum) in the absence of grwoth regulators, on the inoculum with leaves. But the addition of GA3 or kinetin to the medium with different concentrations of glucose strongly inhibited rhizogenesis. Also, the presence of GA3 and kinetin (added together) in the IBA-containing medium significantly suppressed the rooting on the both kinds of inocula. The excision of leaves and supply of IBA (1 mgl-1) promoted rhozogenesis (3 roots/inoculum) on the shoots growing at the lowest concentration of glucse (30 gl-1). |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes | |