| Plant latin name | Zingiber officinale Roscoe |
| Literature code | Zingiber_officinale-Ref-5 |
| Reference | Zheng Y., et al., Acta Physiol Plant 30: 513-519 (2008) |
| Summary | In this study, using the quadratic saturation 310 D-optimal design method, we examined the effect of kinetin (KT), gibberellic acid (GA), and naphthalene acetic acid (NAA) on microrhizome production in ginger. The
effect of GA on rhizome induction was larger than that of KT or NAA. Using simulation and optimality selection for
tissue culture, we found that concentrations of GA, KT, and
NAA of 1.33–2.35, 0.49–0.66, and 0.62 g/l, respectively,
gave a microrhizome weight of over 0.25 g. The optimal
conditions for microrhizome production were 80 g/l
sucrose, 2 × MS macro-elements, and 1 × MS microelements,
with a photoperiod of 24L:0D (light/dark). At the
same time, 100% survival could be achieved on transfer of
the in vitro ginger plantlets with microrhizomes to soil. |
| Objectives | Enhancement of in vitro ginger microrhizome production |
| Materials | 'Tissue cultures of 'Laiwu' ginger plantlets |
| Explant | 'Tissue cultures of 'Laiwu' ginger plantlets |
| Initial culture | Tissue cultures of virus-free ‘Laiwu’ ginger plantlets were
subcultured in MS (Murashige and Skoog 1962) culture
medium containing 2.0 mg/l kinetin (KT), 0.5 mg/l naphthalene
acetic acid (NAA), and 2% (w/v) sucrose for
30 days. The tissue culture plantlets were obtained from
the vegetable tissue culture laboratory of Shandong
Agricultural University College of Horticulture. |
| Shoot multiplication | To screen for the optimal sucrose concentration for microrhizome
induction, the subcultured virus-free ‘Laiwu’
ginger plantlets were separated into individual plantlets and
cut stems (retaining the basal 0.5–1.0 cm of each stem)
were then transferred to MS medium containing 2.0 mg/l
KT, 0.5 mg/l NAA, and various concentrations of sucrose:
20, 50, 80, 110, or 140 g/l.
D310 design was used to find the optimal plant growth
regulator combination (KT, giberrellic acid [GA], NAA)
for microrhizome induction. The plantlets were transferred
to MS medium containing 4 g/l carrageenan, 80 g/l
sucrose, and different plant growth regulator combinations
according to the saturation 310 D-optimal design matrixes. The cultures were incubated at 25 ± 2℃ under
a 12L: 12D (light/dark) photoperiod with a light intensity
of 800 µmol m-2s-1 PPFD for microrhizome induction.
To examine the effect of mineral nutrition on microrhizome
induction, ginger plantlets were incubated on MS
carrageenan medium with different mineral nutrient combinations, and incubated at 25 ± 2℃, 12L: 12D
photoperiod and 800 µmol m-2s-1 PPFD for microrhizome
induction.
The effect of photoperiod on microrhizome induction was
also examined by incubating ginger plantlets in MS culture
medium with 80 g/l sucrose, 2.0 mg/l KT, 0.8 mg/l NAA,
and photoperiods of 0/24, 12/12, 16/8, or 24/0 h at 25 ± 2℃.
The
effect of GA on rhizome induction was larger than that of KT or NAA. Using simulation and optimality selection for
tissue culture, we found that concentrations of GA, KT, and
NAA of 1.33–2.35, 0.49–0.66, and 0.62 g/l, respectively,
gave a microrhizome weight of over 0.25 g. The optimal
conditions for microrhizome production were 80 g/l
sucrose, 2 × MS macro-elements, and 1 × MS microelements,
with a photoperiod of 24L:0D (light/dark). |
| Rooting | |
| Acclimation | Ginger plantlets with microrhizomes
produced using the above in vitro culture conditions
can be successfully transferred to soil, giving survival
rates of up to 100%. |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes | |