Abbas M, et al., Journal of Microbiology, Biotechnology and Food Sciences (JMBFS) 4: 142-148 (2014)
Summary
The present investigation was carried out to highlight an effective protocol for in vitro production of ginger microrhizomes. Microrhizomes were induced at the base of the in vitro derived shoots upon transfer to MS medium containing various concentrations of sucrose (30, 60 and 90 g/L), BAP: 6-benzylaminopurine (3, 6 and 9 mg/L) and grown under varying photoperiodism. In addition MS medium supplemented with 9 mg/L BAP and 60-90 g/L sucrose under 16-h photoperiod within 10 weeks of cultivation were the best conditions for microrhizomes induction. Ginger microrhizomes formation in vitro was found to be controlled by many factors, including the concentrations of BAP and sucrose as well as photoperiodism during culturing period.
Objectives
Effective, reliable and reproducible protocol
for in-vitro production of ginger microrhizomes
Materials
In vitro derived shootlets
Explant
In vitro derived shootlets
Initial culture
Shoot multiplication
Four to five centimeter long of the in vitro derived shootlets were separated from the initial culture and transferred to solid MS medium (Murashige and Skoog 1962 ) supplemented with different concentrations of sucrose (30, 60 and 90 g/L), BAP (3, 6 and 9 mg/L) and 0.7% agar. Each treatment consisted of 5 replicates in addition to the control treatment. The solidified MS cultures media were divided into two groups the first group was incubated under a 16-h/day photoperiod at intensity of 3000 Lux from cool light fluorescent lamps for 10 weeks (two subcultures). The second was maintained in darkness conditions for 10 weeks (two subcultures). All cultures were incubated at 25±1℃. For each combination of previous media, three experiments were carried out consisting of 30 treatments for each 5 replicates under light and darkness conditions. We can concluded that MS medium supplemented with 9 mg/L BAP and 60-90 g/L sucrose under 16-h photoperiod within 10 weeks of cultivation were the best conditions for ginger microrhizomes induction.