Tissue Culture Literation
Plant latin name | Panax ginseng C. A. Mey. |
Literature code | Panax_ginseng-Ref-7 |
Reference | Shoyama Y et al., Planta Medica 54: 155-156 (1988) |
Summary | Embryogenesis of Panax ginseng was induced from young flower buds via callus during a 3 months culture period. Matured embryos could be germinated on the 1/2 Murashige and Skoog's medium supplemented with GA3 (1.4 µM)-BAP (2.2 µM) and 1.5% sucrose. In the medium containing 1.4 µM GA3 and 11.1 µM BAP, a multiple shoot complex having 8 shoots per segment was formed from a single shoot set. On the other hand, the addition of 11.1 µM BAP and 1.4 µM GA3 to the medium stimulated the flower bud formation. For root formation of shoots, the MS medium supplemented with 5.4 µM NAA was the most favourable. Subsequently the plantlet was transferred to vermiculite. |
Objectives | |
Materials | |
Explant | |
Initial culture | |
Shoot multiplication | |
Rooting | |
Acclimation | |
Planting | |
Cultivation conditions | |
Traints of regenerants | |
Ingredients analyzed | |
Extraction | |
Analitical methods | |
Notes |