Comprehensive Medicinal Plant Database

Tissue Culture Literation

Plant latin namePanax ginseng C. A. Mey.
Literature codePanax_ginseng-Ref-7
ReferenceShoyama Y et al., Planta Medica 54: 155-156 (1988)
SummaryEmbryogenesis of Panax ginseng was induced from young flower buds via callus during a 3 months culture period. Matured embryos could be germinated on the 1/2 Murashige and Skoog's medium supplemented with GA3 (1.4 µM)-BAP (2.2 µM) and 1.5% sucrose. In the medium containing 1.4 µM GA3 and 11.1 µM BAP, a multiple shoot complex having 8 shoots per segment was formed from a single shoot set. On the other hand, the addition of 11.1 µM BAP and 1.4 µM GA3 to the medium stimulated the flower bud formation. For root formation of shoots, the MS medium supplemented with 5.4 µM NAA was the most favourable. Subsequently the plantlet was transferred to vermiculite.
Objectives
Materials
Explant
Initial culture
Shoot multiplication
Rooting
Acclimation
Planting
Cultivation conditions
Traints of regenerants
Ingredients analyzed
Extraction
Analitical methods
Notes