Tissue Culture Literation
| Plant latin name | Panax ginseng C. A. Mey. |
| Literature code | Panax_ginseng-Ref-8 |
| Reference | Arya S et al., Plant Cell, Tissue and Organ Culture 34: 157-162 (1993) |
| Summary | Somatic embryos and embryogenic callus were initiated from immature zygotic embryos of ginseng (Panax ginseng C.A. Meyer). These somatic embryos were multiplied by adventitious (secondary and tertiary) embryogenesis and their growth and development were dependent on growth hormones in the medium. Auxins, 2,4-D, NAA, and IAA at 1.0 mg 1-1 were effective in inducing secondary and tertiary somatic embryos, which proliferated directly from the apical or cotyledonary portions of the primary somatic embryos. Single somatic embryos or clusters of embryos developed from the explanted primary embryos. Cytokinin (Kn, BA) inhibited adventitious embryogenesis. Secondary somatic embryos developed to maturation and later regenerated into plantlets in two stage process; firstly elongation of the shoot axes on MS +1.0 mg 1-1 Kn, secondly formation of root on 1.0 mg 1-1 Kn + 1.0 mg 1-1 GA 3 medium. |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |