Tissue Culture Literation
| Plant latin name | Coptis teeta |
| Literature code | Coptis_teeta-Ref-1 |
| Reference | Tandon P and Rathore T.S., Plant Cell, Tissue and Organ Culture 28: 115-117 (1992) |
| Summary | Callus cultures of Coptis teeta were established from hypocotyl segments (excised from aseptically germinating seeds) on Murashige and Skoog (MS) medium containing 2,4-dichlorophenoxyacetic acid(2,4-D) and kinetin. Microshoots were produced within 6-7 weeks of subculturing this callus in 1/2 MS nutrient medium supplemented with kinetin alone. Excised microshoots were rooted in 1/2 MS nutrient medium containing indolebutyric acid (IBA). The complete plantlets were hardened and established. |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |