Comprehensive Medicinal Plant Database

Tissue Culture Literation

Plant latin name Coptis teeta
Literature code Coptis_teeta-Ref-2
Reference Tandon P et al., Indian Journal of Biotechnology 6: 280-282 (2007)
Summary Multiple shoots were produced from axillary buds with rhizome parts from mature plants of Coptis teeta on 1/2 strength Murashige & Skoog's (MS) nutrient salts medium containing 4.42 μM benzylamino purine+0.56 μM indole-3-acetic acid. Isolated shoots were rooted on 1/2 strength MS medium supplemented with 7.35 μM indole-3-butyric acid. Plantlets were also regenerated directly from rhizome segments cultured on Gamborg's medium containing 2.31 μM kinetin+0.56 μM indole-3-acetic acid. Regenerated plantlets were hardened and established in the glass house.
Objectives
Materials
Explant
Initial culture
Shoot multiplication
Rooting
Acclimation
Planting
Cultivation conditions
Traints of regenerants
Ingredients analyzed
Extraction
Analitical methods
Notes