Tissue Culture Literation
| Plant latin name | Rehmannia glutiosa Libosch. f. hueichingensis (Chao et Schih) Hsiao |
| Literature code | Rehmannia_glutinosa_f._hueichingensis-Ref-1 |
| Reference | Xu XH and Davey MR, Plant Cell Reports 2: 55-57 (1983) |
| Summary | Mesophyll protoplasts obtained from leaves of shoot cultures of Rehmannia glutinosa were cultured in Murashige and Skoog (1962) liquid or liquid-over-agar medium containing 2.0 mg L−1 naphthaleneacetic acid and 0.5 mg L−1 benzylamino purine. An amino acid mixture of glutamine, arginine, glycine, and aspartic acid promoted sustained protoplast division, with an average plating efficiency of 27%. Protoplast-derived colonies formed callus which readily regenerated shoots on t ransfer to Murashige and Skoog based agar medium with 2.0 mg L−1 indoleacetic acid and 1.0 mg L−1 benzylamino purine. Leaf explants also showed a marked capacity for shoot regeneration in culture. |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |