Tissue Culture Literation
| Plant latin name | Plantago asiatica Ｌ． |
| Literature code | Plantago_asiatica-Ref-1 |
| Reference | Makowczyńska J and Andrzejewska-Golec E, Acta Soc. Bo t. Pol., 69: 245-250 (2000) |
| Summary | Early stages of direct somatic embryogenesis in Plantago asiatica were observed by light microscopy. In calli of Plantago asiatica two phenomena occurred simultaneously: somatic organogenesis and somatic embryogenesis. |
| Objectives | Induction of somatic embryogenesis in Plantago asiatica L. |
| Materials | Seeds of Plantago asiatica L. from Botanical Garden Faculty of Science, University of Tokyo |
| Explant | Seeds |
| Initial culture | Seeds of Plantago asiatica L. were surface sterilized by immersion in 2.0% sodium hypochlorite for 10 min, then rinsed 3 times in sterile water. Then, they were germinated in aseptic conditions on Murashige and Skoog medium supplemeted with 0.2 mg/l kinetin and 1 mg/l giberellic acid and 0.7% Difco Bacto Agar. The cultures were placed in the dark and after germination they were transferred into light. 4 week-old seedling were used as explants (hypocotyls, roots, cotyledons). |
| Shoot multiplication | For embryogenic callus initiation, explants were placed on the MS medium with 0.5 or 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/l 6-benzylaminopurin (BAP). The best result for callus initiation was observed with the root as explant, and somatic embryos; globular stage embryos and polycotyledonary torpedo stage embryos occurred on the root tissue cultured with 1 mg/l 2,4-D and 0.2 mg/l BAP (passage 0). The resulting calli after 6 weeks of passage 0 were transferred onto agar medium MS with 0.5 mg/l 2,4-D and 0.2 mg/l BAP or 0.2 mg/l α-naphthaleneacetic acid (NAA) and 0.2 mg/l BAP or only 0.5 mg/l BAP-6 weeks of passage 1. In calli of various origin histological study showed globular, heart and aberrant torpedo stage embryos and rhizogenesis was observed. Resulting microshoots had well-shaped chloroplasts, stomata and headed hairs typical for Plantaginaceae family. The calli obtained from the first passage were grown on MS medium supplemented with 0.5 mg/l abcisic acid (ABA) for prevention of aberrations in somatic embryogenesis and 1 mg/l BAP-passage 2-5. In passage 5, many microshoots formed on the callus induced from root. |
| Rooting | |
| Acclimation | |
| Planting | |
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| Traints of regenerants | |
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| Extraction | |
| Analitical methods | |
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