| Plant latin name | Angelica acutiloba Kitagawa |
| Literature code | Angelica_acutiloba-Ref-1 |
| Reference | Watanabe A et al., Plant Cell Reports 18: 187–192 (1998) |
| Summary | Angelica acutiloba, a medicinal plant used as a natural medicine Touki, was clonally propagated through axillary buds in vitro. No substantial differences were found in the random amplified polymorphic DNA (RAPD) pattern between the original A. acutiloba and the plant propagated in vitro, suggesting no changes in the DNA sequences and structure during in vitro propagation. The genetic similarities of several Angelica plants were investigated by restriction fragment length polymorphism (RFLP) and RAPD analyses. The RFLP and RAPD patterns of A. sinensis Diels were substantially different from those of A. acutiloba. Using ten different restriction enzymes, no RFLP was observed in the varieties of A. acutiloba. By RAPD analysis, A. acutiloba varieties can be classified into two major subgroups, i.e., A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyamae Hikino. The varieties of A. acutiloba Kitagawa in Japan and
Angelica spp. in northeast China exhibited a very close genetic relationship.
Angelica acutiloba, a medicinal plant used as a natural medicine Touki, was clonally propagated through axillary buds in vitro. No substantial differences were found in the random amplified polymorphic DNA (RAPD) pattern between the original A. acutiloba and the plant propagated in vitro, suggesting no changes in the DNA sequences and structure during in vitro propagation. The genetic similarities of several Angelica plants were investigated by restriction fragment length polymorphism (RFLP) and RAPD analyses. The RFLP and RAPD patterns of A. sinensis Diels were substantially different from those of A. acutiloba. Using ten different restriction enzymes, no RFLP was observed in the varieties of A. acutiloba. By RAPD analysis, A. acutiloba varieties can be classified into two major subgroups, i.e., A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyamae Hikino. The varieties of A. acutiloba Kitagawa in Japan and
Angelica spp. in northeast China exhibited a very close genetic relationship.
Angelica acutiloba, a medicinal plant used as a natural medicine Touki, was clonally propagated through axillary buds in vitro. No substantial differences were found in the random amplified polymorphic DNA (RAPD) pattern between the original A. acutiloba and the plant propagated in vitro, suggesting no changes in the DNA sequences and structure during in vitro propagation. The genetic similarities of several Angelica plants were investigated by restriction fragment length polymorphism (RFLP) and RAPD analyses. The RFLP and RAPD patterns of A. sinensis Diels were substantially different from those of A. acutiloba. Using ten different restriction enzymes, no RFLP was observed in the varieties of A. acutiloba. By RAPD analysis, A. acutiloba varieties can be classified into two major subgroups, i.e., A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyamae Hikino. The varieties of A. acutiloba Kitagawa in Japan and
Angelica spp. in northeast China exhibited a very close genetic relationship. |
| Objectives | We established an alternative method of in vitro propagation of A. acutiloba and then clarified the molecular genetic relations of the several species of Angelica used as the natural medicine Touki by RFLP and RAPD analyses. |
| Materials | One-year-old plants of A. acutiloba Kitagawa, cultivated in the field at Tsukuba Medicinal Plant Research Station, Natural Institude of Health Science, Tsukuba, Japan, were used to establish the shoot cultures. |
| Explant | The shoots (ca 2 cm length), with leaves and petioles removed, were washed in 75% EtOH for 30 s, rinsed in sterilized water once, surface-sterilized in 2% sodium hypochlorite with Tween 20 (one drop/40 ml) for 10 min and then washed with sterilized water three times.Shoot tips (ca 1 mm height) were excised with a scalpel under a stereo-binocular microscope. |
| Initial culture | Shoot tips were inoculated in 30×150 mm test tubes containing Murashige and Skoog (MS) solid medium (Murashige and Skoog 1962) containing 30 g/l sucrose and 0.2% Gelrite (pH 5.7) supplemented with 0.01 mg/l 1-naphthaleneacetic acid and 0.1 mg/l kinetin, then cultured at 25°C under 16 h light (70 mmol /m/ s)/8 h dark for 8 weeks. |
| Shoot multiplication | The axillary buds formed from the shoot tips were transferred onto hormone-free MS solid medium and repeatedly subcultured under the same conditions to obtain sufficient materials for the experiments. |
| Rooting | For root induction, shoots over 3 cm in height were transferred onto MS solid medium containing 0.5 mg/l indole-3-acetic acid, and cultured under the same conditions as described above. |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes | |