Tissue Culture Literation
| Plant latin name | Polygala tenuifolia Willdenow |
| Literature code | Polygala_tenuifolia-Ref-1 |
| Reference | 尾嵜誠・岡本誉充・奈良女昭・神田博史・藤野廣春・鈴木正一・吉崎正雄・佐竹元吉 植物組織培養12: 97-98 (1995) |
| Summary | Polygala tenuifolia (Onji) is an perennial herb in the family Polygalaceae which grows naturally in east part of Huabei (the North China) and northeastern part of China . The plant is used mainly as an expectorant and tonic. Field-cultivation studies of this plant have so far been reported, however, there is no report on tissue culture. Here tissue culture conditions for the regeneration of plants from the callus which induced from the leaf segment of the seedlings have been reported to establish the vegetative mass propagation of this plant. |
| Objectives | Establishment of vegetative mass propagation method for Polygala tenuifolia |
| Materials | Seeds collected from the plants cultivated in Experimental Station for Medicinal Plant Research, Graduate School, Faculty of Pharmaceutical Sciences for Education (Pharmaceutical Sciences), University of Toyama |
| Explant | Seeds |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |