| Plant latin name
| Prunus armeniaca Linné |
| Literature code
| Prunus_armeniaca-Ref-1 |
| Reference
| Joseph C. G. et al., Plant Growth Regulation 17: 41-46 (1995) |
| Summary
| A prerequisite for most transformation systems is an efficient and reliable method to regenerate phenotypically normal plants. Immature embryos or cotyledons were cultured at three developmental stages (stage 1, 2 and 3, PF = 3, 30-60, and 100, respectively) from two unrelated apricot genotypes, ‘Zard’ and ‘NJA82’. Explants were cultured on MS media supplemented with either BA or TDZ at four concentrations (0, 0.5, 5.0 or 20 μM) and 2,4-D at 0 or 1 μM. Stage 1 embryos cultured on MS medium without growth regulators formed embryoid-like structures. Shoot primordia induction was greatest with stage 2 cotyledons on media containing 5-20 μM TDZ and 1 μM 2,4-D, although shoot morphology was abnormal, especially with the highest level of TDZ. In another factorial experiment, stage 2 cotyledons were cultured on media containing TDZ (0, 5, 7.5, 10, 15 or 20 μM) in combination with either no auxin, 1 μM 2,4-D, 1 μM IBA, or 5 μM IBA. Regeneration percentages of 80% or more were observed on media containing l-5 μM IBA and 5-10 μM TDZ. The medium containing 5 μM IBA and no TDZ exhibited the highest frequency of phenotypically normal plantlet regeneration. |
| Objectives
| Development of a protocol to regenerate apricot |
| Materials
| Embryos were obtained from two genotypes, ‘Zard’, a Central Asian apricot cultivar, and ‘NJA82’, an unrelated selection from the New Jersey Agricultural Experiment Station Tree Fruit Breeding Program. |
| Explant
| Three stages of immature embryo development were utilized: 1) < 1 mm, PF = 3 (38-40 days after anthesis), 2) 5-10 mm, PF = 30-60 (46-48 days after anthesis), and 3) 1.5cm, PF = 100 (58-62 days after anthesis) . |
| Initial culture
| Immature fruit were harvested and surface sterilized with 0.5% sodium hypochlorite containing one drop of Tween-20 for 30 min and rinsed with distilled water. |
| Shoot multiplication
| MS medium containing 5μM IBA exhibited the highest frequency of plantlet regeneration (92 and 100 percent with ‘NJA82’ and‘Zard’, respectively). Transfer of regenerated shoots to WPM with 6 μM 2iP and 2.2 μM BA induced elongation. |
| Rooting
| Subculture on WPM with 10 μM IBA induced only minimal callus, and rooting was observed when the cultures were placed in total darkness for approximately 2 weeks. |
| Acclimation
| Rooted shoots were planted in soilless media, acclimated under intermittent mist for approximately 2 weeks, and later transplanted into the field. |
| Planting
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| Cultivation conditions
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| Traints of regenerants
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| Ingredients analyzed
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| Extraction
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| Analitical methods
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| Notes
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