Tissue Culture Literation
| Plant latin name | Aconitum carmichaeli Debeaux |
| Literature code | Aconitum_carmichaeli-Ref-1 |
| Reference | Hatano K et al., Plant Cell Reports 6:446-448 (1987) |
| Summary | Anthers of Aconitun carmichaeli Debx. were used for callus induction. After the addition of 5ppm 2,4-D and 1ppm kinetin callus induction occurred over a period of 15 weeks. When calluses were subcultured on a medium containing 1 ppm 2,4-D for 12 weeks, embryogenesis occurred. Mature somatic embryos developed normal shoots when transferred to basal medium inoculated with 1 ppm GA and 5 ppm BAP. Rooting occurred after the transfer of shoots to a new medium containing 0.5 ppm IAA and plantlets formed. The transplantation of these was successful and all plants matured during 5 months subsequent cultivation. |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |