Tissue Culture Literation
| Plant latin name | Citrus unshiu Marcowicz |
| Literature code | Citrus_unshiu-Ref-1 |
| Reference | Nito N and Iwamasa M, Plant Cell, Tissue and Organ Culture 20: 137-140 (1990) |
| Summary | In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu Marc.) Nobumasa Nito & Masao Iwamasa Callus was induced from juice vesicles of satsuma mandarin on Murashige & Skoog medium supplemented with ct-naphthaleneacetic acid (NAA), kinetin (K) and gibberellin (GA). Adventitious embryoids arose from the callus tissue on the medium containing 1 mg 1 ~ NAA alone. The embryoids grew into embryos which resulted in a plantlet on medium containing 1 mg 1-1 GA. |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |