The ability of calli induced from leaf disks and immature seeds of Aralia cordata Thunb. to undergo somatic embryogenesis was investigated. Calli of ‘Aichi-bozu’ which were induced by leaf disk culture all produced somatic embryos on solid MS medium (Murashige and Skog, 1962) modified as follows: NH4NO3 (1/4 of original) and 68.435 g･liter-1 (0.2M) sucrose, supplemented with 1.0 mg･liter-1 2,4-D. However, of the calli derived from the leaf disks of ‘Iseshiro’ and ‘Murasaki-menoshiro’ less than 65% regenerated embryos on all media. Of the calli derived from immature seeds (3.0 mm length) of ‘Iseshiro’ and ‘Murasaki-menoshiro’ 100% and 88.9%, respectivery, produced somatic embryos. In the former the calli were induced on the solid MS medium supplemented with 0.5 or 1.0 mg･liter-1 2,4-D, whereas the latter contained 1.0 mg･liter-1 2,4-D. From these results,we founds that in ‘Aichi-bozu’ the callus from leaf disks can produced somatic embryos at a very high frequency on MS medium with a low concentration of NH4NO3 and a high concentration of sucrose, supplemented with 2,4-D, whwewas, in ‘Iseshiro’ and ‘Murasaki-menoshiro’, the calli from immature seeds cultured on MS medium supplemented with 2,4-D produced somatic embryos at high frequency. These somatic embryos grew into complete plants.
Objectives
masspropagation of Arakia cordata using embryogenesis.
Materials
Aralia cordata ‘Aichi-bozu’, ‘Iseshiro’ and ‘Murasaki-menoshiro’obtained from Highland Cold Climate Branch Station in Gumma Horticultural Experiment Station
Explant
leaf and immature seed
Initial culture
‘Aichi-bozu’ (Callus induction from leaf disk) : MS medium with 1/4 of NH4NO3 , 68.435 g･liter-1 sucrose and 0.7% Agar, supplemented with 1.0 mg･liter-1 2,4-D, ‘Iseshiro’ and ‘Murasaki-menoshiro’ (Callus induction from immature seed): MS medium with 30 g･liter-1 sucrose and 0.7% Agar, supplemented with 1.0 mg･liter-1 2,4-D.
Shoot multiplication
（somatic embryo induction): MS medium with 30 gliter-1sucrose and 0.7% Agar
Rooting
（growth of somatic embryo): MS medium with 30 gliter-1sucrose and 0.7% Agar