Tissue Culture Literation
| Plant latin name | Zizyphus jujuba Miller var. spinosa Hu ex H. F. Chou |
| Literature code | Zizｙphu_ jujuba-Ref-1 |
| Reference | Gu X. F. and Zhang J. R., Plant Cell Report 23: 775-779 (2005) |
| Summary | The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves were explanted onto Woody Plant Medium and maintained initially in the dark. The plant growth regulator thidiazuron (TDZ) was effective in stimulating shoot regeneration from leaf explants of Zhanhua winter jujube. The highest efficiency of shoot formation was observed with a 20-day culture in the dark on WPM containing 4.54 μM TDZ and 2.85 μM indoleacetic acid (IAA). The regenerated shoots were transferred to MS medium containing 0.89 μM benzyladenine and 5.77 μM gibberellic acid for growth. When the shoots were about 2 cm in height, they were transferred to Nitsch medium supplemented with 1.14 μM IAA and 2.46 μM indolebutyric acid to induce rooting. This system of adventitious shoot production from leaf explants of adult plants could be useful for the genetic engineering and polyploidization of winter jujube. |
| Objectives | establishment of efficient in vitro system with high regeneration frequency for Zhanhua winter jujube. |
| Materials | Zhanhua winter jujube (Zizyphus jujuba Mill.) were obtained from 50- to 55-year-old elite plants growing at the Zhanhua Winter Jujube Institute |
| Explant | dormant buds |
| Initial culture | The single-node cuttings were sterilized by immersion in 70% (v/v) ethanol for 30 s, followed by treatment with HgCl2 (0.1%, w/v) for 8 min. The cuttings were then rinsed three or four times with sterile distilled water and inoculated vertically on MS medium supplemented with 11.4μM GA3 and 0.5 μM BA. |
| Shoot multiplication | Adventitious shoot formation from cultured 10-day-old leaf explants of Zhanhua winter jujube were obtained on WPM medium containing 4.54 μM TDZ and 2.85 μM IAA. |
| Rooting | About 90% of shoots rooted on Nitsch basal medium containing 1.14 μM IAA, 2.46 μM IBA and 2% sucrose. |
| Acclimation | Rooted plantlets were transplanted into plastic pots containing autoclaved vermiculite and soil (1/1, v/v). |
| Planting | After 30 days, the surviving plants were transplanted into the field. |
| Cultivation conditions | |
| Traints of regenerants | |
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