Tissue Culture Literation
| Plant latin name | Chrysanthemum indicum Linné |
| Literature code | Chrysanthemum_indicum-Ref-1 |
| Reference | Fukai S et al, Euphytica 54: 201-204 (1991) |
| Summary | The shoot tips of Chrysanthemum morifolium (syn. Dendranthema grandiflorum) and related species native to Japan were cryopreserved using preculture for 2 days, slow cooling (0.2 ° C/min) until - 40 ° C with 10% dimethyl sulphoxide and 3% glucose prior to immersion into LN2 and rapid thawing. High survival rates were observed in 3 cultivars of chrysanthemum, 12 species and 2 interspecific hybrids, and slightly low survival rates in 3 species. The shoot regeneration rates of the frozen shoot tips varied from 9.4 to 100% depending on species. Shoot tips of chrysanthemum showed high viability even after a storage of 8 months in LN2. The thawed chrysanthemum shoot tips grew and flowered normally in a greenhouse under natural conditions. |
| Objectives | This study was carried out to clarify the applicability of this freezing method to the related species of chrysanthemum. |
| Materials | Shoot tips were excised from mother plants growing in a greenhouse under night break (22:00--02:00). |
| Explant | |
| Initial culture | The excised shoot tips were precultured on MS medium supplemented with 0.1 mg/1 BA, 1.0 mg/l NAA, 20 g/1 sucrose, 8 g/l agar and 5% (v/v) DMSO at 25 ° C under a constant fluorescent light at 19/xmol/s/m 2 for 2 days. |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |