Tissue Culture Literation
| Plant latin name | Rehmannia glutiosa Libosch. var. purpurea Makino |
| Literature code | Rehmannia_glutinosa_var._purpurea-Ref-2 |
| Reference | Shoyama Y et al., Planta Medica 48: 124-128 (1983) |
| Summary | Shoot tips of Rehmannia glutinosa LIBOSCHITZ var. purpurea MAKINO were grown in medium supplemented with 1ppm indole-3-acetic acid (IAA) and 1ppm 6-benzylaminopurine (BAP). Subculture of the shoot in medium containing BAP (5 ppm) or a combination of gibberellic acid A3 (GA) and BAP resulted in multiple shoot formation. Subsequent transfer of these shoots to hormone-free medium resulted in rooting. |
| Objectives | Clonal multiplication method of Rehmannia glutinosa from shoot tips and the successful establishment of the plantlets obtained in vitro into soil |
| Materials | |
| Explant | root which were bedded in sterilized vermiculite |
| Initial culture | Portions of the shoot were removed, sterilized and washed with sterilized water. The shoot tips were dissected from the shoots with the aid of a binocular microscope into sections 0.5-1 mm in length. The basal medium consisted of Murashige and Skoog (MS) salts supplemented by auxin (IAA), cytokinins and GA in concentrations and combinations. The growth of the shoot tip was most successful when cultured on IAA and BAP (1 ppm each) containing medium; therefore, this became the medium routinely used in the initial stage. |
| Shoot multiplication | After the initial stage, the cultured shoot was transferred to MS medium supplemented with kinetin, BAP and GA and cultured. With 5 ppm BAP, a shoot segment produced a multiple shoot complex of approximately 20 shoots per segment. |
| Rooting | Rooting occurred when the proliferated shoot clump was cut into units consisting of 2 petioles attached to a basal stem portion and these shoot sections were transferred to hormone free MS medium. |
| Acclimation | |
| Planting | Subsequently, the rooted plantlets were successfully transferred to vermiculite. |
| Cultivation conditions | |
| Traints of regenerants | The plants grew to be similar to their parental strains when transferred to soil and cultivated. It is theoretically possible to obtain more than 2 x 1010 plants per year. |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |