Tissue Culture Literation
| Plant latin name | Paeonia lactiflora Pallas |
| Literature code | Paeonia_lactiflora-Ref-2 |
| Reference | 米田ら, 生薬学雑誌, 29(1) 6-9 (1975) |
| Summary | Embryo culture of Paeonia albiflora PALLAS was made for knowing the state of embryo in the germinating term. Embryoes were taken out from sterilized seeds, and embedded on Nitsch's medium, and were incubated. As the result, the following properties became clear. The temperature desired for radicle elongation of embryo is about 20〜25°C. The radicle starts elongation in 7 − 10 days after embedded on but seed cold storaged takes more. The dormancy of hypocotyl is caused by embryo, and is broken by 10〜100 μg／embryo of gibberellic acid. |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |