Tissue Culture Literation
| Plant latin name | Rheum palmatum Linne |
| Literature code | Rheum_palmatum-Ref-2 |
| Reference | Kaneko K. et al., Shoyakugaku Zasshi 40(4): 401-405 (1986) |
| Summary | Meristem tips of Rheum palmatum L. grown on a paper bridge placed on a MS-medium containing 8 mg/l of naphthalene acetic acid (NAA) and 2.56 mg/l of benzylaminopurine (BAP) were induced to form shoots which gave rapidly proliferating shoots within 14 days of transfer to a medium containing 4 mg/l of NAA and 7.56 mg/l of BAP. Subsequent transfer of the shoots to a BAP free medium resulted in root development within 3 weeks. The rooted plantlets were readily established in peat pots with vermiculite within three weeks and were subsequently cultivated with soil for 6 months (the highest being 30 cm after 6 months). |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |