Tissue Culture Literation
| Plant latin name | Dioscorea japonica Thunb. |
| Literature code | Dioscorea_japonica-Ref-2 |
| Reference | 松原, 石原, 岡山大学報, (72) 19-26 (1988) |
| Summary | Production of the virus free lines by the shoot apex culture and the vegetative propagation of the line obtained in some cultivars of the Chinese yam (Dioscorea sp.) were studied. Shoot apices cultured on MS medium containing 0.01 mg/l both of NAA and BA showed shoot and root growth. The best results were obtained when explants were cultured on the basal medium containing 0.1 mg/L NAA and 0.01 mg/l BA. For the stem explants, 2.4% of them formed adventitious buds, and 9.6% regenerated root. |
| Objectives | |
| Materials | |
| Explant | |
| Initial culture | |
| Shoot multiplication | |
| Rooting | |
| Acclimation | |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |