Tissue Culture Literation
| Plant latin name | Phellodendron amurense Ruprecht |
| Literature code | Phellodendron_amurense-Ref-2 |
| Reference | M. A. K. Azad & S. Yokota & F. Begum & N. Yoshizawa, In Vitro Cell.Dev.Biol.-Plant (2009) 45:441–449 |
| Summary | "Somatic embryogenesis and subsequent plant regeneration were established from hypocotyl and internode explants collected from in vitro-grown seedlings and in vitro- proliferated shoots, respectively. Somatic embryogenesis was significantly influenced by the types of auxin and cytokinin. Friable calluses with somatic embryos developed well in Murashige and Skoog basal (MS) medium supplemented with 0.8–8.8 μM 6-benzylaminopurine (BA) and 2.0–8.0 μM 2,4-dichlorophexoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA). The maximal frequency of embryogenic callus and somatic embryo formation were obtained when the MS medium was amended with 8.8 μM BA and 4.0 μM 2,4-D. The best embryo germination occurred in a hormone-free 1/2-MS medium. The highest percentage of shoot proliferation was observed in embryo- genic calluses in MS medium containing 2.0 μM BA and 1.0 μM NAA. In vitro-grown shoots were rooted in MS medium with 0.5–2.0 μM indole-3-butyric acid. Regenerants were transferred to vermiculite and successfully established under an ex vitro environment in garden soil. " |
| Objectives | The aim of the investigation reported here was to successful plant regeneration through the somatic embryogenesis of P. amurense. |
| Materials | Immature fruits of P. amurense Rupr. were collected from the Medicinal Plant Garden of Kumamoto University, Kumayaku, Japan.the seeds were surface-sterilized with 70.0% EtOH for 3 min, 3.0% sodium hypochlorite (NaOCl) solution for 20 min and riced with sterilized distilled water. |
| Explant | Seeds were germinated in MS medium supplemented with 2.2 μM BA. |
| Initial culture | The cultured seeds germinated within 3 wk and gave rise to shoots that developed two–three nodes 5–6 wk later. Shoots were then propagated by subculturing single-node cuttings at 4-wk intervals in MS medium with 2.0 μM BA. |
| Shoot multiplication | embryogenic callus and somatic embryo formation were obtained when the MS medium was amended with 8.8 μM BA and 4.0 μM 2,4-D.The highest percentage of shoot proliferation was observed in embryo- genic calluses in MS medium containing 2.0 μM BA and 1.0 μM NAA. |
| Rooting | In vitro-grown shoots were rooted in MS medium with 0.5–2.0 μM indole-3-butyric acid. |
| Acclimation | Regenerants were transferred to vermiculite and successfully established under an ex vitro environment in garden soil. |
| Planting | |
| Cultivation conditions | |
| Traints of regenerants | The surviving plantlets did not show any detectable variations in morphological or growth characteristics from the donor plants. |
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| Extraction | |
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| Notes |