Comprehensive Medicinal Plant Database

Tissue Culture Literation

Plant latin nameGentiana scabra Bunge,Gentiana manshurica Kitagawa
Literature codeGentiana_scabra-Ref-2
ReferenceShih-Hung Huang, Dinesh Chandra Agrawal, Fang-Sheng Wu and Hsin-Sheng Tsay, Huang et al. Botanical Studies: 55:56 ( 2014)
Summary"Shoot apices of six weeks old in vitro grown G. scabra plants were used as explants for the in vitro propagation. Induction of multiple shoots (9.1/explant) was achieved on the culture of shoot apices on half strength Murashige and Skoog’s basal medium (MSBM) containing 2.0 mg/L−1 6-benzylaminopurine (BA), 3% sucrose and 0.9% Difco agar. In vitro shoots induced profuse rooting on half strength of MSBM supplemented with 0.1 mg/L−1 1-naphthaleneacetic acid (NAA), 3% sucrose and 0.3% gelrite. A two-stage ventilation closure procedure during the in vitro culture, and transparent sachet technique enhanced the survival rate of G. scabra plantlets to 96% in the greenhouse. Tissue culture plants flowered after 5 months of transfer to pots. "
ObjectivesThe main objective of the study was to develop a simple and an efficient in vitro propagation method of G. scabra with a high survival rate of plantlets.
MaterialsIn vitro plants of G. scabra were obtained from the Taiwan Sugar Corporation, Taiwan
ExplantShoot apices (1.0 cm) excised from the six weeks old in vitro plants were used as explants
Initial cultureInduction of multiple shoots (9.1/explant) was achieved on the culture of shoot apices on half strength Murashige and Skoog’s basal medium (MSBM) containing 2.0 mg/L−1 6-benzylaminopurine (BA), 3% sucrose and 0.9% Difco agar.
Shoot multiplication
RootingIn vitro shoots induced profuse rooting on half strength of MS medium supplemented with 0.1 mg/L−1 NAA, 3% sucrose and 0.3% gelrite.
AcclimationPlantlets were briefly treated with 0.1% Benlate (a systemic fungicide) solution (DuPont, Wilmington, DE) and transplanted into plastic pots con- taining a mixture of peatmoss:perlite:vermiculite (2:1:1 v/v). For acclimatization, each potted plant was covered with a transparent polyethylene sachet
Planting
Cultivation conditions
Traints of regenerants
Ingredients analyzed
Extraction
Analitical methods
Notes