Tissue Culture Literation
| Plant latin name | Gardenia jasminoides Ellis |
| Literature code | Gardenia_jasminoides-Ref-2 |
| Reference | P.S. George, et al., Plant Cell Reports 13: 59-62 (1993) |
| Summary | An efficient clonal multiplication system was developed for in vitro propagation of crocin -producing Gardenia jasminoides Ellis plants. MS medium containing BAP 1 mg/l and IBA 1 mg/l resulted in multiple shoot initiation at the rate of 21 shoots per explant in 60 d of culture. Transfer of the microshoots into liquid MS medium supplemented with BAP (5 mg/l) with two subcultures of 15 d duration in the same medium resulted in 400土 25 shoots per explant. Efficient rooting was achieved in MS medium supplemented with NAA (5 mg /1). The in vitro raised plants were hardened in a greenhouse and transplanted to the field successfully. The method described will be useful for rapid multiplication of Gardenia for commercial exploitation. |
| Objectives | This communication presents a highly efficient method for clonal multiplication of elite plants of G. jasminoides by axillary bud culture |
| Materials | Stem cuttings of Gardenia jasminoides Ellis were collected from 15 year old field grown plants from Wayanad，Kerala，India. |
| Explant | The shoot tips，1.5 cm long，with two axil1ary buds，were excised and the terminal bud was removed. The explants were washed thoroughly in running tap water for 30 min.，surface sterilised with 0.15% (w/v) mercuric chloride for 6 min. and washed with sterile distilled water 3 times. |
| Initial culture | MS medium containing BAP 1 mg/l and IBA 1 mg/l resulted in multiple shoot initiation at the rate of 21 shoots per explant in 60 d of culture. |
| Shoot multiplication | Transfer of the microshoots into liquid MS medium supplemented with BAP (5 mg/l) with two subcultures of 15 d duration in the same medium resulted in 400土 25 shoots per explant. |
| Rooting | Efficient rooting was achieved in MS medium supplemented with NAA (5 mg/l). |
| Acclimation | The rooted plantlets established well upon transfer to pots in a greenhouse. |
| Planting | Subsequent transfer of potted plants to the field was successful. |
| Cultivation conditions | |
| Traints of regenerants | |
| Ingredients analyzed | |
| Extraction | |
| Analitical methods | |
| Notes |